Digital Nucleic Acid Amplification on a Slipchip
نویسندگان
چکیده
This presentation will describe a recently developed SlipChip based microfluidic platform to perform digital nucleic acid amplification in a simple and low-cost format. Samples can be introduced into the SlipChip by pipetting without the use of complex instruments, and thousands of nanoliter volume compartments can be generated by simple slipping. A “yes-or-no” digital readout of end-point fluorescence was used to detect the presence of nucleic acid in each compartment, and the concentration of nucleic acid in the original sample was calculated. The SlipChip may find applications for detecting bacterial DNA by PCR and isothermal amplification, and quantifying HIV viral load by RT-PCR.
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